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K-ACETAK Megazyme ACETIC ACID (ACETATE KINASE FORMAT) ASSAY PROCEDURE FOR AUTO-ANALYSER
This kit contains 550 assays.
The most widely used method for enzymatic quantification of acetic acid is that employing acetyl-coenzyme A synthetase (ACS). However, this method is based on the use of an indicator reaction catalysed by L-malate dehydrogenase that is in permanent equilibrium, and therefore a non-stoichiometric increase in absorbance is observed from the acetate present in the sample. Thus, a slightly non-linear response to increasing acetate concentration is observed upon calibration, resulting in poor R2 values. In addition (depending on the supplier) reagent prepared for auto-analyser applications can have very limited on-machine stability, due to rapidly increasing blank absorbance values. To overcome these issues, Megazyme developed this alternative acetic acid kit, based on the enzyme acetate kinase (AK), especially for the auto-analyser user. This reagent has improved on-machine stability, gives excellent linear calibration curves, and results in a stoichiometric change in absorbance due to the acetic acid present in the sample.